Journal of Chemistry and Biochemistry

Analytical Method Development for the Spectrophotometric Determination of Sulfamethoxazole in Bulk Drug and Pharmaceutical Preparation
Zaman Sahb Mehdi

Abstract
A spectrophotometric method for the determination of Sulfamethaxazole (SMX) in bulk drug and in solid dosage was developed. The method was based on the condensation reaction between primary aromatic amine group present in drug with aromatic aldehyde, vanillin, to produce a yellow color. The resulting Schiff’s base shown maximum absorption at 372nm. The reaction product is stable. The reaction was carried out in acetic acid and in 0.1M perchloric acid medium. The Beer’s law was obeyed in the ranges 1.5–40 mg/L with the acceptable correlation coefficient r = 0.9993. The detection limit and limit of detection were 0.19 and 0.24 mg/L of SMX, respectively. The method was successfully applied to the determination of this compound in pharmaceutical formulation.

Full Text: PDF     DOI: 10.15640/jcb.v3n1a5